OLYMPUS

Total internal reflection fluorescence microscopy (TIRF) makes use of specific optics to produce illumination light only at the 50-100 nm range at the interface of the slide, massively reducing out of focus light and improving the ability to detect fluorescent molecules. Because of its low light intensity and high spatial resolution, it is a key technique in live-cell imaging. Designed for membrane dynamics, single molecule detection, and colocalization experiments, the IXplore TIRF microscope system offers simultaneous multicolor TIRF imaging for up to 4 colors with high stability. The Total Internal Reflection Fluorescence (TIRF) technique is the ideal method for observations of cells close to the coverslip surface.

Designed for deep imaging in biological specimens, the multiphoton microscope helps reveal how cells function and interact within living tissue.
Two-photon excitation microscopy (also called Multiphoton) is a fluorescence imaging technique that allows imaging of living tissue up to about one millimeter in thickness, Unlike traditional fluorescence microscopy, in which the excitation wavelength is shorter than the emission wavelength, two-photon excitation requires simultaneous excitation by two photons with longer wavelength than the emitted light. Two-photon excitation can be a superior alternative to confocal microscopy due to its deeper tissue penetration, efficient light detection, and reduced photobleaching.